Human Secukinumab (Secukinumab) ELISA Kit

Cat.#:

JL58267

Regulatory Status:

RUO

Alternative:

Secukinumab

Detection Method:

ELISA

Assay Type:

Indirect

Detection Range:

0.78-50ng/mL

Sensitivity:

0.47ng/mL

Specificity:

This kit recognizes Human Secukinumab in samples. No significant cross-reactivity or interference between Human Secukinumab and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| Secukinumab

Secukinumab is a human IgG1κ monoclonal antibody used for the treatment of psoriasis, ankylosing spondylitis, and psoriatic arthritis.It binds to the protein interleukin (IL)-17A.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	50	25	12.5	6.25	3.12	1.56	0.78	0
OD	2.23	1.79	1.27	0.8	0.51	0.32	0.22	0.12
Corrected OD	2.11	1.67	1.15	0.68	0.39	0.2	0.1	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of HumanSecukinumab spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-104	94
Plasma(n=8)	90-106	97
Cell Culture Supernates(n=8)	95-110	99

| Linearity

Samples were spiked with high concentrations of HumanSecukinumab and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-98	88-96	90-110
1 : 2	Average (%)	92	90	95
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	95	91	105